產(chǎn)品概述

基質(zhì)金屬蛋白酶（MMPs）是鋅依賴性降解細(xì)胞外基質(zhì)蛋白的內(nèi)肽酶家族中的一員。它以酶原（pro-MMPs）形式被分泌，可以激活多種蛋白酶或是在有機(jī)汞存在的條件下發(fā)揮作用。MMP的活性調(diào)控在組織重建、炎癥、腫瘤生成和轉(zhuǎn)移中起重要作用。單核細(xì)胞、巨噬細(xì)胞、嗜中性粒細(xì)胞、角化細(xì)胞、成纖維細(xì)胞、成骨細(xì)胞、軟骨細(xì)胞、骨骼肌星狀細(xì)胞、血管內(nèi)皮細(xì)胞和各種腫瘤細(xì)胞都可以分泌酶原 MMP -9。TGF-β 1，IL-1β，TGF-α，PDGF-AB，TNF-α和IL-1α可以使酶原MMP-9表達(dá)上調(diào)。 MMP-9 與炎癥反應(yīng)、組織構(gòu)建、傷口愈合、生長因子和細(xì)胞因子過程有關(guān)。它的表達(dá)與伴隨胰腺癌生成的結(jié)締組織、與人乳腺癌細(xì)胞轉(zhuǎn)移到淋巴結(jié)和骨巨噬細(xì)胞瘤入侵局部導(dǎo)管相關(guān)。 MMP-9 在齦溝液、齒齦炎、牙周炎患者的唾液可能表達(dá)比較高。

產(chǎn)品原理

本實(shí)驗(yàn)采用雙抗體夾心ELISA法。抗人MMP-9單抗包被于酶標(biāo)板上，標(biāo)本和標(biāo)準(zhǔn)品中的MMP-9會與單抗結(jié)合形成免疫復(fù)合物，游離的成分被洗去；加入生物素化的抗人MMP-9抗體，生物素化抗人MMP-9抗體與酶標(biāo)板上結(jié)合的標(biāo)準(zhǔn)品或樣本中的MMP-9結(jié)合而形成免疫復(fù)合物，游離的成分被洗去；加入辣根過氧化物酶標(biāo)記的親合素，親合素與生物素特異性結(jié)合，游離的成分被洗去。加入顯色底物（顯色劑），若反應(yīng)孔中有MMP-9，辣根過氧化物酶會使無色的顯色劑變藍(lán)，加終止液變黃。在450nm處測OD值， MMP-9濃度與OD₄₅₀值之間呈正相關(guān)，可通過繪制標(biāo)準(zhǔn)曲線求出標(biāo)本中MMP-9濃度。

產(chǎn)品性能

1. 靈敏度：最小可測人MMP-9達(dá)30pg/ml 。

2. 重復(fù)性：板內(nèi)、板間變異系數(shù)均<10%。

產(chǎn)品組分及儲存條件

試驗(yàn)所需自備儀器和試劑:

1. 酶標(biāo)儀(450nm檢測波長濾光片，570nm或630nm校正波長濾光片)。

2. 高精度加液器及一次性吸頭：0.5-10μl，2-20μl，20-200μl，200-1000μl。

3. 37℃恒溫箱、蒸餾水或去離子水、坐標(biāo)紙。

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Zhang SY et al.Adipocyte-derivedLysophosphatidylcholine Activates Adipocyte and Adipose TissueMacrophage Nod-LikeReceptor Protein 3 Inflammasomes Mediating Homocysteine-InducedInsulinResistance.EBioMedicine, 2018. Read more (Pubmed : 29735414)

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