產(chǎn)品概述
IL-1包含兩種不同基因的編碼的蛋白，IL-1α和IL-1β，但是這兩種亞型具有25%的氨基酸序列同源性，并且它們可識(shí)別相同的細(xì)胞受體。已知的分泌IL-1的細(xì)胞有成骨細(xì)胞，單核細(xì)胞，巨噬細(xì)胞，角質(zhì)形成細(xì)胞，Kupffer細(xì)胞，肝細(xì)胞，胸腺和唾液腺上皮，雪旺氏細(xì)胞，成纖維細(xì)胞和神經(jīng)膠質(zhì)細(xì)胞（少突神經(jīng)膠質(zhì)，星形膠質(zhì)細(xì)胞和小膠質(zhì)細(xì)胞）。IL-1α和IL-1β都是由31KDa的前體蛋白合成。在不同的物種中，成熟的IL-1α的氨基酸序列有60%到70%的保守性，已發(fā)現(xiàn)人的IL-1可以作用于鼠的細(xì)胞系。
產(chǎn)品原理
本實(shí)驗(yàn)采用雙抗體夾心ELISA法。抗大鼠IL-1α單抗包被于酶標(biāo)板上，同時(shí)加入標(biāo)本、標(biāo)準(zhǔn)品和生物素化的抗大鼠IL-1α抗體，標(biāo)本、標(biāo)準(zhǔn)品中的IL-1α?xí)c加入于孔中的生物素化的抗大鼠IL-1α抗體及包被于酶標(biāo)板上的單抗結(jié)合，形成免疫復(fù)合物，游離的成分被洗去；加入辣根過氧化物酶標(biāo)記的親合素，親合素與生物素特異性結(jié)合，游離的成分被洗去。加入顯色底物（顯色劑），若反應(yīng)孔中有IL-1α，辣根過氧化物酶會(huì)使無色的顯色劑變藍(lán)，加終止液變黃。在450nm處測OD值， IL-1α濃度與OD450值之間呈正相關(guān)，可通過繪制標(biāo)準(zhǔn)曲線求出標(biāo)本中IL-1α濃度。
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